DMEM/F12 Basal Medium Size:6 x 500 mL

DMEM/F12 Basal Medium Size:6 x 500 mLDefined Biosciences DMEM F12 is a fully defined basal medium for use in the culture of a wide range of mammalian cells. DMEM F12 is a 1: 1 mixture of DMEM and Hams F 12, buffered with HEPES and sodium bicarbonate for more comprehensive buffering, and substituted with L alanyl L glutamine in place of glutamine for improved cell health and product stability. DMEM F12 should be used with other growth factors, sera, or additives (NOT INCLUDED) depending

defined formulation designed for the feeder-free maintenance and expansion of human pluripotent stem cells (PSCs) in an undifferentiated state

Defined Bioscience’s HiDef® PBS/EDTA is a defined reagent for protein-free adherent cell dissociation

as its composition is optimized specifically for HiDef-B8 use

carrier- free

HiDef-ITS is provided as a 100x supplement in Earle’s Balanced Salt Solution (EBSS) without phenol red

human (also provided by Defined Bioscience as Cat # LSR-101)

Sample purity is >95% as confirmed by SDS-PAGE

including Corning® (#10-092)

Gibco™ (#11330)

sufficient for 500 mL of medium

FGF2 (tuna) is recombinantly expressed in E

With its unique blend of DMEM and Ham's F-12 media